Removal of interference by erythromycin, phenazopyridine, and methenamine mandelate in the Porter-Silber reaction.
نویسنده
چکیده
Erythromycin, phenazopyridine, methenamine mandelate, acetazolamide, and ascorbic acid reportedly interfere with the Porter-Silber reaction in the determination of urinary 17-hydroxycorticosteroids. Sodium bisulfite, added to the urine after hydrolysis with glucuronidase, removed almost all nonsteroidal interferences in urine from healthy adults medicated with the first three drugs, but such studies proved that acetazolamide and ascorbic acid do not interfere with the Porter--Silber reaction, whether or not sodium bisulfite is added.
منابع مشابه
Interference by formaldehydogenic drugs in the quantitative determination of urinary hydroxyindoleacetic acid.
The administration of methenamine mandelate (Mandelamine) to normal individuals and patients with carcinoid resulted in a marked reductionin apparent urinary hydroxyindoleaceticacid (HIAA) as determined by the quantitative nitroso-naphthol method. Mandelamine, methenamine, and formaldehyde inhibited the normal color development in vitro. The interfering reaction is dependent on an acid pH and i...
متن کاملDetermination of urinary formaldehyde following administration of methenamine salts.
C TJRRENT COLORIMETRIC METHODS for the measurement of formaldehyde in the presence of methenamine salts appear misleading. The principal problems with these technics include the release of formaldehyde (1, 2) from intact methenamine and the nonspecificity of the reaction producing color with other compounds present in urine. However, a modification of the method of Tannenbaum and Bricker (3) ap...
متن کاملThe effect of methenamine mandelate (mandelamine) on determination of urinary estriol.
The administration of methenamine mandelate (Mandelamine) to pregnant women causes the levels of estriol in their urines to be unmeasurably low when acid hydrolysis is used. When enzyme hydrolysis replaces acid hydrolysis, normal estriol values are obtained. This result is attributed to the formaldehyde released by acid from hexamethylenetetramine, a constituent of the drug. The formaldehyde re...
متن کاملDetermination of placental estriol in urine by gas-liquid chromatography, with equilenin as internal standard.
Placental estriol in urine can be determined by rapid enzymatic hydrolysis, extraction with neutral XAD-2 resin, and quantitation by gas-liquid chromatography, with equilenin as the internal standard. Glucose, methenamine mandelate, hydrochlorothiazide, phenolphthalein, or salicylate do not interfere. Analytical recovery of estriol added to urine was 92-108%. Day-to-day precison (CV), establish...
متن کاملRapid radioimmunoassay of total urinary estriol.
We describe a radioimmunoassay for total urinary estriol in pregnancy. A 25-mul aliquot of the urine specimen is acid hydrolyzed, neutralized, and diluted before assay. We use rabbit antisera against estriol-6-(O-carboxymethyl)oxime/bovine serum albumin and ammonium sulfate precipitation at room temperature. Results are unaffected by glucose or methenamine mandelate, a urinary tract antiseptic....
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید
ثبت ناماگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید
ورودعنوان ژورنال:
- Clinical chemistry
دوره 23 11 شماره
صفحات -
تاریخ انتشار 1977